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crb2 rabbit polyclonal antibody  (Bioss)


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    Structured Review

    Bioss crb2 rabbit polyclonal antibody
    Crb2 Rabbit Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crb2+rabbit+polyclonal+antibody/CRB2+Polyconal+Antibody/pmc12076484-252-47-52
    Average 90 stars, based on 2 article reviews
    crb2 rabbit polyclonal antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: CRB2 Depletion Induces YAP Signaling and Disrupts Mechanosensing in Podocytes
    Article Snippet: .. After washing the cells for 5 min twice, they were incubated with the following primary antibodies at 4°C overnight: nephrin rabbit polyclonal antibody (1:200, Thermo Fisher Scientific, PA5-20330), synaptopodin mouse monoclonal antibody (1:200, Progen Biotechnik, 61094), FAK1mouse monoclonal antibody (1:100, Millipore Sigma, 05-537), CRB2 rabbit polyclonal antibody (1:100, BIOSS, BS-14046R), TRPC6 mouse monoclonal antibody (1:100, Abcam, 105845), and at room temperature for one hour: paxillin rabbit monoclonal antibody (1:1200, Cell Signaling, 50195) in 1% BSA. .. After washing 3 to 5 times with 1X PBS or 1X PBST, cells were incubated in the following secondary antibodies for one hour at room temperature in dark: Alexa Fluor Plus 647 donkey anti-mouse (1:800) (Thermo Fisher, A32787), Cy5 donkey anti-rabbit (1:400, Jackson ImmunoResearch, 711-175-152), Alexa Fluor 555/488 donkey anti-mouse and donkey anti-rabbit (1:1000, ThermoFisher, A-31570 and A-21206), TRITC-phalloidin (1:500, Sigma Aldrich, FAK100), Alexa Fluor 488/555-phalloidin (1:1000, ThermoFisher, A12379 and A30106) and DAPI (1:800 or 1:2000).

    Article Title: CRB2 Depletion Induces YAP Signaling and Disrupts Mechanosensing in Podocytes
    Article Snippet: .. After washing the cells for 5 min twice, they were incubated with the following primary antibodies at 4°C overnight: nephrin rabbit polyclonal antibody (1:200, Thermo Fisher Scientific, PA5–20330, RRID:AB_11153994), synaptopodin mouse monoclonal antibody (1:200, Progen Biotechnik, 61094, RRID:AB_1543006), FAK1 mouse monoclonal antibody (1:100, Millipore Sigma, 05–537, RRID:AB_2173817), CRB2 rabbit polyclonal antibody (1:100, BIOSS, BS-14046R, Ref. ( 49 )), TRPC6 mouse monoclonal antibody (1:100, Abcam, 105845, RRID:AB_10901396, Ref. ( 50 )), and at room temperature for one hour: paxillin rabbit monoclonal antibody (1:1200, Cell Signaling Technologies, MA, 50195) in 1% BSA. .. After washing 3 to 5 times with 1X PBS or 1X PBST, cells were incubated in the following secondary antibodies for one hour at room temperature in dark: Alexa Fluor Plus 647 donkey anti-mouse (1:800, Thermo Fisher Scientific, A32787, RRID:AB_2762830), Cy5 donkey anti-rabbit (1:400, Jackson ImmunoResearch, 711-175-152, RRID:AB_2340607), Alexa Fluor 555/488 donkey anti-mouse and donkey anti-rabbit (1:1000, Thermo Fisher Scientific, A-31570, RRID:AB_2536180 and A-21206, RRID:AB_2535792), TRITC-phalloidin (1:500, Sigma Aldrich, FAK100), Alexa Fluor 488/555-phalloidin (1:1000, Thermo Fisher Scientific, A12379, RRID:AB_2535792 and A30106) and DAPI (1:800 or 1:2000).



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